Detection of porcine rotavirus in stools by a latex agglutination test

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

agglutination . Detection of rotavirus by latex

I was very interested to read the letter of Dr Waitkins et al on the importance of Streptococcus milleri. We too isolate the organism from cases of empyema from time to time and would agree about the importance of S milleri in this context. Although we welcome this reminder about the pathogenicity of the organism, there is one aspect of the letter that could be misleading: S milleri apparently ...

Development of a slide latex agglutination test for rotavirus antigen detection.

The aim of this study was to optimize the conditions for the passive adsorption of polyclonal antibody onto plain surface polystyrene latex particles and its performance in a slide latex agglutination test for rotavirus antigen detection. Cleaning of latex particles by washing through repetitive centrifuging, decanting and resuspending in distilled water was adequate in removing surfactants fro...

Development of a Latex Agglutination Method for Diagnosis of Rotavirus Infections

Background: Rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. Since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included Electron Microscopy (EM) and enzyme immunoassay. These methods, however, are expensive and not readily available everywhere. We have developed a technique which can be used for ...

Rapid diagnosis of rotavirus gastroenteritis by a commercial latex agglutination test.

The Rotalex test, a commercial latex agglutination test for rotavirus, was compared with direct electron microscopy (EM) and the Rotazyme test I, a commercial enzyme immunoassay, for detection of rotavirus in stools of children and neonates. For initial stool specimens from 265 children (less than 3 years old) with diarrhea, the Rotalex test had a sensitivity of 81.7% and specificity of 99.5% c...